Frailty and elderly in urology: Is there an impact on post-operative complications?

INTRODUCTION: Frailty used as predictive tool is still not carried out in daily practice, although many studies confirm the great clinical importance of the frailty syndrome in surgical outcomes. There is no standardized method of measuring the physiological reserves of older surgical patients. The aim of this study was to analyze a cohort of older urological patients according to various frailty indices, in order to evaluate whether they are predictors of post-operative complications after urological procedures. MATERIAL AND METHODS: This is a prospective observational study on 78 consecutive older ( 6570 years) patients, subjected to major urological (both endoscopic and 'open surgical') procedures. Frailty was defined according to the Edmonton Frail Scale. Several risk models and biochemical parameters were evaluated. Post-operative outcomes were surgical and medical complications, mortality and rehospitalisation within 3 months. RESULTS: An overall prevalence of frailty of 21.8% was found. Patients with complications were frailer than those without complications (univariate analysis), considering both total patients (p = 0.002) and endoscopic (p = 0.04) and 'open surgical' patients (p = 0.013). However, in multivariate analysis, a significant correlation was not found between all frailty indices tested and the risk of major complications. Limitation of the study: the small sample size (lack of statistical power), although this is a prospective study focused on older urological patients. CONCLUSIONS: New urology-tailored pre-operative assessment tools may prove beneficial when calculating the risks/benefits of urological procedures, so that objective data can guide surgical decision- making and patient counselling. Further large clinical studies specifically focusing on elderly in urology will be needed

Special enzymes, like "Easter Eggs" with wonderful functions for biotechnology

We guess you know very well what an Easter Egg is in a video game: a special surprise that was hidden in a secret area or a bonus object. We discovered [Morra et al., 2016a, check Bibliography for info and link to this original scientific paper and to other papers cited] a similar “Easter Egg” in a protein that functions in a very useful bacterium, and this suggested us how proteins and enzymes can contribute to a bio-sustainable future. But first let us introduce you the scientific background and the context. Continua a leggere..

AQP5 is expressed in type-B intercalated cells in the collecting duct system of the rat, mouse and human kidney.

We screened human kidney-derived multipotent CD133+/CD24+ ARPCs for the possible expression of all 13 aquaporin isoforms cloned in humans. Interestingly, we found that ARPCs expressed both AQP5 mRNA and mature protein. This novel finding prompted us to investigate the presence of AQP5 in situ in kidney. We report here the novel finding that AQP5 is expressed in human, rat and mouse kidney at the apical membrane of type-B intercalated cells. AQP5 is expressed in the renal cortex and completely absent from the medulla. Immunocytochemical analysis using segment- and cell type-specific markers unambiguously indicated that AQP5 is expressed throughout the collecting system at the apical membrane of type-B intercalated cells, where it co-localizes with pendrin. No basolateral AQPs were detected in type-B intercalated cells, suggesting that AQP5 is unlikely to be involved in the net trans-epithelial water reabsorption occurring in the distal tubule. An intriguing hypothesis is that AQP5 may serve an osmosensor for the composition of the fluid coming from the thick ascending limb. Future studies will unravel the physiological role of AQP5 in the kidney

Enzimi speciali come "Easter Eggs" con funzioni meravigliose per le biotecnologie

Immaginiamo che sappiate bene cosa sia un "Easter Egg" in un videogioco: una sorpresa speciale nascosta in un'area segreta o un oggetto bonus. Abbiamo scoperto [Morra et al, 2016, cercalo nelle referenze, troverai il link all’articolo scientifico originale] un "Easter Egg" simile in una proteina che funziona in un batterio molto utile, e questo ci ha suggerito come proteine ed enzimi possano contribuire a un futuro bio-sostenibile. Ma prima lasciate che vi presentiamo il background scientifico e il contesto. Continua a leggere..

Enzimi speciali come "Easter Eggs" con funzioni meravigliose per le biotecnologie

Immaginiamo che sappiate bene cosa sia un "Easter Egg" in un videogioco: una sorpresa speciale nascosta in un'area segreta o un oggetto bonus. Abbiamo scoperto [Morra et al, 2016, cercalo nelle referenze, troverai il link all’articolo scientifico originale] un "Easter Egg" simile in una proteina che funziona in un batterio molto utile, e questo ci ha suggerito come proteine ed enzimi possano contribuire a un futuro bio-sostenibile. Ma prima lasciate che vi presentiamo il background scientifico e il contesto. Continua a leggere..

Single-Scale Natural SUSY

We consider the prospects for natural SUSY models consistent with current data. Recent constraints make the standard paradigm unnatural so we consider what could be a minimal extension consistent with what we now know. The most promising such scenarios extend the MSSM with new tree-level Higgs interactions that can lift its mass to at least 125 GeV and also allow for flavor-dependent soft terms so that the third generation squarks are lighter than current bounds on the first and second generation squarks. We argue that a common feature of almost all such models is the need for a new scale near 10 TeV, such as a scale of Higgsing or confinement of a new gauge group. We consider the question whether such a model can naturally derive from a single mass scale associated with supersymmetry breaking. Most such models simply postulate new scales, leaving their proximity to the scale of MSSM soft terms a mystery. This coincidence problem may be thought of as a mild tuning, analogous to the usual mu problem. We find that a single mass scale origin is challenging, but suggest that a more natural origin for such a new dynamical scale is the gravitino mass, m_{3/2}, in theories where the MSSM soft terms are a loop factor below m_{3/2}. As an example, we build a variant of the NMSSM where the singlet S is composite, and the strong dynamics leading to compositeness is triggered by masses of order m_{3/2} for some fields. Our focus is the Higgs sector, but our model is compatible with a light stop (with the other generation squarks heavy, or with R-parity violation or another mechanism to hide them from current searches). All the interesting low-energy mass scales, including linear terms for S playing a key role in EWSB, arise dynamically from the single scale m_{3/2}. However, numerical coefficients from RG effects and wavefunction factors in an extra dimension complicate the otherwise simple story.Comment: 32 pages, 3 figures; version accepted by JHE

A Novel Two-Component Signaling System Facilitates Uropathogenic Escherichia coli\u27s Ability to Exploit Abundant Host Metabolites

wo-component signaling systems (TCSs) are major mechanisms by which bacteria adapt to environmental conditions. It follows then that TCSs would play important roles in the adaptation of pathogenic bacteria to host environments. However, no pathogen-associated TCS has been identified in uropathogenic Escherichia coli (UPEC). Here, we identified a novel TCS, which we termed KguS/KguR (KguS: α-ketoglutarate utilization sensor; KguR: α-ketoglutarate utilizationregulator) in UPEC CFT073, a strain isolated from human pyelonephritis. kguS/kguR was strongly associated with UPEC but was found only rarely among other E. coli including commensal and intestinal pathogenic strains. An in vivo competition assay in a mouse UTI model showed that deletion of kguS/kguR in UPEC CFT073 resulted in a significant reduction in its colonization of the bladders and kidneys of mice, suggesting that KguS/KguR contributed to UPEC fitness in vivo. Comparative proteomics identified the target gene products of KguS/KguR, and sequence analysis showed that TCS KguS/KguR and its targeted-genes,c5032 to c5039, are encoded on a genomic island, which is not present in intestinal pathogenicE. coli. Expression of the target genes was induced by α-ketoglutarate (α-KG). These genes were further shown to be involved in utilization of α-KG as a sole carbon source under anaerobic conditions. KguS/KguR contributed to the regulation of the target genes with the direct regulation by KguR verified using an electrophoretic mobility shift assay. In addition, oxygen deficiency positively modulated expression of kguS/kguR and its target genes. Taken altogether, this study describes the first UPEC-associated TCS that functions in controlling the utilization of α-ketoglutarate in vivo thereby facilitating UPEC adaptation to life inside the urinary tract

The Double Cover of the Icosahedral Symmetry Group and Quark Mass Textures

We investigate the idea that the double cover of the rotational icosahedral symmetry group is the family symmetry group in the quark sector. The icosahedral (A5) group was previously proposed as a viable family symmetry group for the leptons. To incorporate the quarks, it is highly advantageous to extend the group to its double cover, as in the case of tetrahedral (A4) symmetry. We provide the basic group theoretical tools for flavor model-building based on the binary icosahedral group I' and construct a model of the quark masses and mixings that yields many of the successful predictions of the well-known U(2) quark texture models.Comment: 10 pages, references added, typos in up quark mass matrix correcte

Out Of This World Supersymmetry Breaking

We show that in a general hidden sector model, supersymmetry breaking necessarily generates at one-loop a scalar and gaugino mass as a consequence of the super-Weyl anomaly. We study a scenario in which this contribution dominates. We consider the Standard Model particles to be localized on a (3+1)-dimensional subspace or ``3-brane'' of a higher dimensional spacetime, while supersymmetry breaking occurs off the 3-brane, either in the bulk or on another 3-brane. At least one extra dimension is assumed to be compactified roughly one to two orders of magnitude below the four-dimensional Planck scale. This framework is phenomenologically very attractive; it introduces new possibilities for solving the supersymmetric flavor problem, the gaugino mass problem, the supersymmetric CP problem, and the mu-problem. Furthermore, the compactification scale can be consistent with a unification of gauge and gravitational couplings. We demonstrate these claims in a four-dimensional effective theory below the compactification scale that incorporates the relevant features of the underlying higher dimensional theory and the contribution of the super-Weyl anomaly. Naturalness constraints follow not only from symmetries but also from the higher dimensional origins of the theory. We also introduce additional bulk contributions to the MSSM soft masses. This scenario is very predictive: the gaugino masses, squark masses, and $A$ terms are given in terms of MSSM renormalization group functions.Comment: 42 pages, LateX, references added, corrections added as Eqs. (43, 53

Pseudonatural Inflation

We study how to obtain a sufficiently flat inflaton potential that is natural from the particle physics point of view. Supersymmetry, which is broken during inflation, cannot protect the potential against non-renormalizable operators violating slow-roll. We are therefore led to consider models based on non-linearly realized symmetries. The basic scenario with a single four-dimensional pseudo Nambu Goldstone boson requires the spontaneous breaking scale to be above the Planck scale, which is beyond the range of validity of the field theory description, so that quantum gravity corrections are not under control. A nice way to obtain consistent models with large field values is to consider simple extensions in extra-dimensional setups. We also consider the minimal structures necessary to obtain purely four-dimensional models with spontaneous breaking scale below M_P; we show that they require an approximate symmetry that is supplemented by either the little-Higgs mechanism or supersymmetry to give trustworthy scenarios.Comment: 30 pages, 2 figures. v2: minor changes, ref. added, accepted for JCA